Transcriptomics Sandbox

type access

  • Operating System:

  • Shell:

  • Editor:

  • Package Manager:

  • Programming Language:

type access

  • Operating System:

  • Shell:

  • Editor:

  • Package Manager:

  • Programming Language:

type access

  • Operating System:

  • Shell:

  • Editor:

  • Package Manager:

  • Programming Language:

In this app you will find transcriptomics related courses you can learn from, and datasets and tools you can work with for your own research/learning purposes. The included materials and tools are organized by the Health Data Science sandbox.

Most items of this sandbox are currently based on Rstudio Server. Rstudio is a web-based integrated development environment for R programming language, including R Markdown, code, and data. Typically, each item includes a dedicated webpage with additional information, guides, and material.

Available Items

Items are periodically added to this app and can be chosen from the menu. Each item can be a course, a setup to work with specific software, a research example and comes with all necessary packages installed, eventual notebooks with computer code and explanations, and a dedicated webpage with additional material (notes, slides, recordings, ...).

The available tools are:

Tool Name

Description

Links

Programming Language

RNAseq in
Rstudio

Rstudio session with common bulk and single cell RNAseq packages such as DESeq2, Seurat and clusterProfiler.

Webpage

R, Rstudio

RNAseq CLI

JupyterLab session within a mamba environment containing common RNAseq tools, ideal to create your own pipelines using snakemake

Python, Snakemake

Cirrocumulus

Cirrocumulus is an interactive visualization tool for large-scale single-cell genomics data.

Webpage

Python, JavaScript

Packages for RNAseq in Rstudio

A few R packages have been installed in order to work with RNAseq data. Other packages and dependencies might be installed but are not shown here.

CRAN

Bioconductor

Remotes

BiocManager

GenomeInfoDb

seurat-wrappers

tidyverse

clusterProfiler

seurat-data

RColorBrewer

DOSE

annotables

pheatmap

org.Hs.eg.db

seurat-disk

ggrepel

org.Mm.eg.db

cowplot

org.Dm.eg.db

Seurat

pathview

patchwork

DEGreport

sctransform

tximport

DESeq2

AnnotationHub

Signac

ensembldb

factoextra

apeglm

FactoMineR

ggnewscale

NMF

rhdf5

hexbin

slingshot

statmod

gprofiler2

Goplot

vsn

ggpubr

airway

ggsci

Courses

The available courses are:

Course Title

Course Code

Description

Links

Programming Language

Introduction to
bulk RNAseq
analysis

Intro_to_bulkRNAseq

A 3-day course to introduce bulk RNAseq analysis, from data alignment to bioinformatics analysis

Webpage

R, Bash, Nextflow

Introduction to
single cell RNAseq
analysis

Intro_to_scRNAseq_R

A 2-day course to introduce single cell RNAseq analysis in R

Webpage

R

Note

Course materials will automatically be downloaded unless you import a folder called Intro_to_bulkRNAseq or Intro_to_scRNAseq_R, respectively, in the job submission page.

Save your work

Everything saved in the /work folder will be saved in your personal folder after you finish your job in UCloud.

Additional options

Before submitting the app, you can choose the amount of resources you need. Additionally, you can add folders so that they will be visible when using the app. Adding folders is useful if:

  • You want to use a folder containing your own data and code, with which you want to perform analysis with the Transcriptomics tools of a course/module.

  • You want to continue working on the material from a previous session of the Transcriptomics Sandbox. In such a case, add the folder containing the material using the Add folder optional parameter.